Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus.
Identifieur interne : 000043 ( Main/Exploration ); précédent : 000042; suivant : 000044Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus.
Auteurs : Xiaogang Liu [République populaire de Chine] ; Yan Luo [République populaire de Chine] ; Hongkun Wu [République populaire de Chine] ; Wanpeng Xi [République populaire de Chine] ; Jie Yu [République populaire de Chine] ; Qiuyun Zhang [République populaire de Chine] ; Zhiqin Zhou [République populaire de Chine]Source :
- Gene [ 1879-0038 ] ; 2016.
English descriptors
- KwdEn :
- Citrus sinensis (chemistry), Citrus sinensis (enzymology), Citrus sinensis (genetics), Flavonoids (biosynthesis), Flavonoids (chemistry), Garcinia cambogia (chemistry), Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Genome, Plant, Methylation, Multigene Family, Organ Specificity, Phylogeny, Plant Proteins (classification), Plant Proteins (genetics), Plant Proteins (metabolism), Protein O-Methyltransferase (classification), Protein O-Methyltransferase (genetics), Protein O-Methyltransferase (metabolism).
- MESH :
- chemical , biosynthesis : Flavonoids.
- chemistry : Citrus sinensis, Flavonoids, Garcinia cambogia.
- chemical , classification : Plant Proteins, Protein O-Methyltransferase.
- enzymology : Citrus sinensis.
- genetics : Citrus sinensis, Plant Proteins, Protein O-Methyltransferase.
- chemical , metabolism : Plant Proteins, Protein O-Methyltransferase.
- Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Genome, Plant, Methylation, Multigene Family, Organ Specificity, Phylogeny.
Abstract
The O-methylation of various secondary metabolites is mainly catalyzed by S-adenosyl-l-methionine (SAM)-dependent O-methyltransferase (OMT) proteins that are encoded by the O-methyltransferase gene family. Citrus fruits are a rich source of O-methylated flavonoids that have a broad spectrum of biological activities, including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. However, little is known about this gene family and its members that are involved in the O-methylation of flavonoids and their regulation in Citrus. In this study, 58 OMT genes were identified from the entire Citrus sinensis genome and compared with those from 3 other representative dicot plants. A comprehensive analysis was performed, including functional/substrate predictions, identification of chromosomal locations, phylogenetic relationships, gene structures, and conserved motifs. Distribution mapping revealed that the 58 OMT genes were unevenly distributed on the 9 citrus chromosomes. Phylogenetic analysis of 164 OMT proteins from C.sinensis, Arabidopsis thaliana, Populus trichocarpa, and Vitis vinifera showed that these proteins were categorized into group I (COMT subfamily) and group II (CCoAOMT subfamily), which were further divided into 10 and 2 subgroups, respectively. Finally, digital gene expression and quantitative real-time polymerase chain reaction analyses revealed that citrus OMT genes had distinct temporal and spatial expression patterns in different tissues and developmental stages. Interestingly, 18 and 11 of the 27 genes predicted to be involved in O-methylation of flavonoids had higher expression in the peel and pulp during fruit development, respectively. The citrus OMT gene family identified in this study might help in the selection of appropriate candidate genes and facilitate functional studies in Citrus.
DOI: 10.1016/j.gene.2015.09.048
PubMed: 26407870
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000085
- to stream PubMed, to step Curation: 000085
- to stream PubMed, to step Checkpoint: 000085
- to stream Ncbi, to step Merge: 001D19
- to stream Ncbi, to step Curation: 001D19
- to stream Ncbi, to step Checkpoint: 001D19
- to stream Main, to step Merge: 000043
- to stream Main, to step Curation: 000043
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus.</title>
<author><name sortKey="Liu, Xiaogang" sort="Liu, Xiaogang" uniqKey="Liu X" first="Xiaogang" last="Liu">Xiaogang Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China; Post-doctoral Station of Horticulture, Southwest University, Chongqing 400715, China. Electronic address: lxgg16853@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China; Post-doctoral Station of Horticulture, Southwest University, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Luo, Yan" sort="Luo, Yan" uniqKey="Luo Y" first="Yan" last="Luo">Yan Luo</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: wodeaily7@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Hongkun" sort="Wu, Hongkun" uniqKey="Wu H" first="Hongkun" last="Wu">Hongkun Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: 1003925334@qq.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xi, Wanpeng" sort="Xi, Wanpeng" uniqKey="Xi W" first="Wanpeng" last="Xi">Wanpeng Xi</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: xwp1999@zju.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yu, Jie" sort="Yu, Jie" uniqKey="Yu J" first="Jie" last="Yu">Jie Yu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: yujie1978@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Qiuyun" sort="Zhang, Qiuyun" uniqKey="Zhang Q" first="Qiuyun" last="Zhang">Qiuyun Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: swuzqy@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Zhiqin" sort="Zhou, Zhiqin" uniqKey="Zhou Z" first="Zhiqin" last="Zhou">Zhiqin Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: zqzhou2013@swu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26407870</idno>
<idno type="pmid">26407870</idno>
<idno type="doi">10.1016/j.gene.2015.09.048</idno>
<idno type="wicri:Area/PubMed/Corpus">000085</idno>
<idno type="wicri:Area/PubMed/Curation">000085</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000085</idno>
<idno type="wicri:Area/Ncbi/Merge">001D19</idno>
<idno type="wicri:Area/Ncbi/Curation">001D19</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001D19</idno>
<idno type="wicri:Area/Main/Merge">000043</idno>
<idno type="wicri:Area/Main/Curation">000043</idno>
<idno type="wicri:Area/Main/Exploration">000043</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus.</title>
<author><name sortKey="Liu, Xiaogang" sort="Liu, Xiaogang" uniqKey="Liu X" first="Xiaogang" last="Liu">Xiaogang Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China; Post-doctoral Station of Horticulture, Southwest University, Chongqing 400715, China. Electronic address: lxgg16853@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China; Post-doctoral Station of Horticulture, Southwest University, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Luo, Yan" sort="Luo, Yan" uniqKey="Luo Y" first="Yan" last="Luo">Yan Luo</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: wodeaily7@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Hongkun" sort="Wu, Hongkun" uniqKey="Wu H" first="Hongkun" last="Wu">Hongkun Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: 1003925334@qq.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xi, Wanpeng" sort="Xi, Wanpeng" uniqKey="Xi W" first="Wanpeng" last="Xi">Wanpeng Xi</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: xwp1999@zju.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yu, Jie" sort="Yu, Jie" uniqKey="Yu J" first="Jie" last="Yu">Jie Yu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: yujie1978@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Qiuyun" sort="Zhang, Qiuyun" uniqKey="Zhang Q" first="Qiuyun" last="Zhang">Qiuyun Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: swuzqy@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Zhiqin" sort="Zhou, Zhiqin" uniqKey="Zhou Z" first="Zhiqin" last="Zhou">Zhiqin Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: zqzhou2013@swu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715</wicri:regionArea>
<wicri:noRegion>Chongqing 400715</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Gene</title>
<idno type="eISSN">1879-0038</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (chemistry)</term>
<term>Citrus sinensis (enzymology)</term>
<term>Citrus sinensis (genetics)</term>
<term>Flavonoids (biosynthesis)</term>
<term>Flavonoids (chemistry)</term>
<term>Garcinia cambogia (chemistry)</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Genome, Plant</term>
<term>Methylation</term>
<term>Multigene Family</term>
<term>Organ Specificity</term>
<term>Phylogeny</term>
<term>Plant Proteins (classification)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Protein O-Methyltransferase (classification)</term>
<term>Protein O-Methyltransferase (genetics)</term>
<term>Protein O-Methyltransferase (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Flavonoids</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
<term>Flavonoids</term>
<term>Garcinia cambogia</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Plant Proteins</term>
<term>Protein O-Methyltransferase</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus sinensis</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term>
<term>Plant Proteins</term>
<term>Protein O-Methyltransferase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term>
<term>Protein O-Methyltransferase</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Developmental</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Genome, Plant</term>
<term>Methylation</term>
<term>Multigene Family</term>
<term>Organ Specificity</term>
<term>Phylogeny</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The O-methylation of various secondary metabolites is mainly catalyzed by S-adenosyl-l-methionine (SAM)-dependent O-methyltransferase (OMT) proteins that are encoded by the O-methyltransferase gene family. Citrus fruits are a rich source of O-methylated flavonoids that have a broad spectrum of biological activities, including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. However, little is known about this gene family and its members that are involved in the O-methylation of flavonoids and their regulation in Citrus. In this study, 58 OMT genes were identified from the entire Citrus sinensis genome and compared with those from 3 other representative dicot plants. A comprehensive analysis was performed, including functional/substrate predictions, identification of chromosomal locations, phylogenetic relationships, gene structures, and conserved motifs. Distribution mapping revealed that the 58 OMT genes were unevenly distributed on the 9 citrus chromosomes. Phylogenetic analysis of 164 OMT proteins from C.sinensis, Arabidopsis thaliana, Populus trichocarpa, and Vitis vinifera showed that these proteins were categorized into group I (COMT subfamily) and group II (CCoAOMT subfamily), which were further divided into 10 and 2 subgroups, respectively. Finally, digital gene expression and quantitative real-time polymerase chain reaction analyses revealed that citrus OMT genes had distinct temporal and spatial expression patterns in different tissues and developmental stages. Interestingly, 18 and 11 of the 27 genes predicted to be involved in O-methylation of flavonoids had higher expression in the peel and pulp during fruit development, respectively. The citrus OMT gene family identified in this study might help in the selection of appropriate candidate genes and facilitate functional studies in Citrus.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Xiaogang" sort="Liu, Xiaogang" uniqKey="Liu X" first="Xiaogang" last="Liu">Xiaogang Liu</name>
</noRegion>
<name sortKey="Luo, Yan" sort="Luo, Yan" uniqKey="Luo Y" first="Yan" last="Luo">Yan Luo</name>
<name sortKey="Wu, Hongkun" sort="Wu, Hongkun" uniqKey="Wu H" first="Hongkun" last="Wu">Hongkun Wu</name>
<name sortKey="Xi, Wanpeng" sort="Xi, Wanpeng" uniqKey="Xi W" first="Wanpeng" last="Xi">Wanpeng Xi</name>
<name sortKey="Yu, Jie" sort="Yu, Jie" uniqKey="Yu J" first="Jie" last="Yu">Jie Yu</name>
<name sortKey="Zhang, Qiuyun" sort="Zhang, Qiuyun" uniqKey="Zhang Q" first="Qiuyun" last="Zhang">Qiuyun Zhang</name>
<name sortKey="Zhou, Zhiqin" sort="Zhou, Zhiqin" uniqKey="Zhou Z" first="Zhiqin" last="Zhou">Zhiqin Zhou</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000043 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000043 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:26407870 |texte= Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26407870" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
This area was generated with Dilib version V0.6.25. |